700
Industrial Park Drive
Alabaster, AL 35007
1-800-227-0651 or 205-663-2494
Fax 1-800-229-1004 or 205-663-0756
Orders:
Technical Questions:
General
Information:
Preparation
This product is prepared by solvent extraction of fresh heart
tissue, followed by purification of the cardiolipin by high-pressure
column chromatography. It is 34 times less soluble in absolute
ethanol and 6 times less soluble in acetone than cardiolipin
prepared by the precipitation method. Reactivity for this
product has been demonstrated in the VDRL diagnostic test
for syphilis.
Schlame, M., D. Rua, and M.L. Greenberg. (2000). The
biosynthesis and functional role of cardiolipin. Prog
Lipid Res 39:257-88.
Cable, M.B., J. Jacobus, and G.L. Powell. (1978). Cardiolipin:
a stereospecifically spin-labeled analogue and its specific
enzymic hydrolysis. Proc Natl Acad Sci U S A 75:1227-31.
Powell, G.L. and J. Jacobus. (1974). The nonequivalence
of the phosphorus atoms in cardiolipin. Biochemistry 13:4024-6.
Courtade, S.A. and J.M. McKibbin. (1965). The Composition
of Beef Heart Cardiolipins Isolated by Solvent and Chromatographic
Fractionation. Lipids 6, 260-65.
Fatty Acid Content of Cardiolipin
*Note: Results expressed as area percentage
by FAME - GC/FID
