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CERTIFICATE OF ANALYSIS #840046
Brain Phosphatidylinositol-4,5-bisphosphate, tri-ammonium salt
Molecular weight†: 1098.19
Physical form: Chloroform:Methanol:Water 66:30:4
Storage Freezer: (-20C)
Manufacture date: October 16, 2000
Expiration date: August 16, 2001
Lot No.: BPIP2(4,5)-15
† Based on 1-Stearoyl-2-Arachidonoyl-sn-Glycero-3-Phosphoinositol-4,5-bisphosphate, tri-ammonium salt
Structure

Product Specifications
 
ANALYSIS
SPECIFICATION
RESULTS
Physical Examination
Clear and near colorless solution which contains no foreign material.
Pass
TLC
Ninhydrin spray, negative
Iodine, 1 spot
Phosphorus spray, positive
Charring, positive
Water dip, 1 spot
Relative Rf consistent with structure.

All Pass
HPLC
Retention time of product peak matches that of the reference standard.

Purity = NLT 99% AUC

Pass

99.8% AUC

Gas Chromatography
Fatty acid methyl ester profile of material is consistent with structure.

(NOT SPECIFICATIONS)
18:0 33 area %
18:1 9 area %
20:4 33 area %

18:0 = 35.6%
18:1 = 14.9%
20:4 = 34.2%
Electrospray MS
(negative mode)
[M-3NH4+2H]- = 1046 ± 1amu
[M-3NH4+H]2- = 522 ± 1 amu
[M-3NH4+2H]- = 1045.6 amu
[M-3NH4+H]2- = 522.3 amu
Calcium ion content
[Ca]2+ NMT 2000 ppm
[Ca]2+< 100 ppm
Concentration (by Phosphorus UV/vis)
Information Only
4.9 mg/ml