Synthetic
Products |
Cationic
Transfection Reagents |
| 1,2-Diacyl-sn-Glycero-3-Ethylphosphocholine |
Purity >99% |
Ethyl DPPC as a Transfection Agent: Effect of SonoPorationTM on Transfection Rates
FluoroGene is a new class of synthetic delivery vehicles with a
fluorinated core that efficiently complexes genes into stable submicron-sized
particles. SonoPoration enhances gene expression and localization
with ultrasound. In these in vivo studies FluoroGene (made
with Avanti's Ethyl DPPC) and SonoPoration were used to deliver
the CAT gene via IM and IV injections in mice. SonoPoration preferentially
increased gene expression in tissues that were exposed to ultrasound,
and IV injection of FluoroGene caused widespread CAT gene expression
with high levels of expression in kidney, in spleen, and in muscle.
FluoroGene works synergistically with SonoPoration, and levels of
gene expression appear to be higher than with other synthetic delivery
vehicles.*
*Unger, E.C., "FluoroGeneTM and SonoPorationTM Gene Delivery" (1997) Presented at the Cambridge Healthtech
Institute's 4th Annual Meeting, Oct 12-14, 1997, by ImaRx Pharmaceutical
Corporation.
References:
- Edwin Pozharski and Robert C. MacDonald (2003)
Lipoplex Thermodynamics: Determination of DNA-Cationic Lipoid Interaction Energies
Biophys. J. 85: 3969-3978.
- Rumiana Koynova and Robert C. MacDonald (2003)
Mixtures of Cationic Lipid O-Ethylphosphatidylcholine with Membrane Lipids and
DNA: Phase Diagrams
Biophys. J. 85: 2449-2465.
- Edwin Pozharski and Robert C. MacDonald (2002)
Thermodynamics of Cationic Lipid-DNA Complex Formation as Studied by Isothermal
Titration Calorimetry
Biophys. J. 83: 556-565.
- Yury S. Tarahovsky, Vera A. Rakhmanova, Richard M. Epand, and
Robert C. MacDonald (2002)
High Temperature Stabilization of DNA in Complexes with Cationic Lipids
Biophys. J. 82: 264-273.
- Rosenzweig, H.S., V.A. Rakhmanova, T.J. McIntosh, and R.C.
MacDonald. (2000).
O-Alkyl dioleoylphosphatidylcholinium compounds: the effect of varying alkyl
chain length on their physical properties and in vitro DNA transfection activity.
Bioconjug Chem 11:306-13.
- Kennedy, M.T., E.V. Pozharski, V.A. Rakhmanova, and R.C. MacDonald.
(2000).
Factors governing the assembly of cationic phospholipid-DNA complexes.
Biophys J 78:1620-33.
-
Tarahovsky, Y.S., A.L. Arsenault, R.C. MacDonald,
T.J. McIntosh, and R.M. Epand. (2000). Electrostatic Control
of Phospholipid Polymorphism.
Biophys J 79:3193-3200.
-
MacDonald, R.C., G.W. Ashley, M.M. Shida,
V.A. Rakhmanova, Y.S. Tarahovsky, D.P. Pantazatos, M.T. Kennedy,
E.V. Pozharski, K.A. Baker, R.D. Jones, H.S. Rosenzweig, K.L.
Choi, R. Qiu, and T.J. McIntosh. (1999).
Physical and biological
properties of cationic triesters of phosphatidylcholine.
Biophys J 77:2612-29.
-
MacDonald, R.C., V.A. Rakhmanova, K.L. Choi,
H.S. Rosenzweig, and M.K. Lahiri. (1999).
O-ethylphosphatidylcholine:
A metabolizable cationic phospholipid which is a serum-compatible
DNA transfection agent.
J Pharm Sci 88:896-904.
-
Matsumura, J.S., R. Kim, V.P. Shively, R.C.
MacDonald, and W.H. Pearce. (1999).
Characterization of vascular
gene transfer using a novel cationic lipid.
J Surg Res 85:339-45.
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