Avanti Polar Lipids

Natural Products

Lipids by Extraction

Brain

Product	Catalog Number
Total Extract*	131101	Product Data
Polar Extract**	141101	Product Data

Brain Lipid Extract	Total Extract	Polar Extract
Brain Lipid Extract	Cat. No. 131101	Cat. No. 141101
Component	Percent/Wt.	Percent/Wt.
Phosphatidylethanolamine	16.7	33.1
Phosphatidylserine	10.6	18.5
Phosphatidylcholine	9.6	12.6
Phosphatidic Acid	2.8	0.8
Phosphatidylinositol	1.6	4.1
Other	58.7	30.9

Egg

Product	Catalog Number
Total Extract* (60% Lecithin)	241601	Product Data
95% Lecithin	131601	Product Data

Egg Lipid Extract	Total Extract
Egg Lipid Extract	Cat. No. 241601
Component	Percent/Wt.
Phosphatidylethanolamine	11.01
Phosphatidylinositol	1.10
Phosphatidylcholine	72.70
Lyso Phosphatidylinositol	2.07
Others, incs. Neutral Lipids	13.12

Escherichia coli

Product	Catalog Number
Total Extract*	100500	Product Data
Polar Extract**	100600	Product Data

E-coli Lipid Extract	Total Extract	Polar Extract
E-coli Lipid Extract	Cat. No. 100500	Cat. No. 100600
Component	Percent/Wt.	Percent/Wt.
Phosphatidylethanolamine	57.5	67.0
Phosphatidylglycerol	15.1	23.2
Cardiolipin	9.8	9.8
Other	17.6	0.0

Total e.Coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is portioned against deionized water and the chloroform phase is concentrated.

Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.

Avanti does not perform any analytical procedures on these extract.

References:

Newman, M.J. & Wilson, T.H., "Solubilization and Reconsitution of the Lactose Transport System from Escherichia coli" (1980) J. Biol. Chem. 255, 10583-10586.
Kagawa, Y. & Rackeer E., "Partial Resolution of the Enzymes Catalyzing Oxidative Phosphorylation", (1971) J. Biol. Chem. 246, 5477-5487.

Heart

Product	Catalog Number
Total Extract*	171201	Product Data
Polar Extract**	171204	Product Data

Heart Lipid Extract	Total Extract	Polar Extract
Heart Lipid Extract	Cat. No. 171201	Cat. No. 171204
Component	Percent/Wt.	Percent/Wt.
Phosphatidylethanolamine	6.8	13.6
Phosphatidylinositol	2.5	1.0
Cardiolipin	2.3	1.7
Phosphatidylcholine	5.4	8.6
Phosphatidic Acid	1.1	0.6
Neutral Lipid	49.8	57.7
Other	32.1	16.8

Liver

Product	Catalog Number
Total Extract*	181104	Product Data
Polar Extract**	181108	Product Data

Liver Lipid Extract	Total Extract	Polar Extract
Liver Lipid Extract	Cat. No. 181104	Cat. No. 181108
Component	Percent/Wt.	Percent/Wt.
Cholesterol	7	5
Phosphatidylethanolamine	22	26
Phosphatidylinositol	8	9
Phosphatidylcholine	42	42
Lyso Phosphatidylinositol	1	1
Others, incs. Neutral Lipids	21	17

Soybean

Product	Catalog Number
Total Extract* (20% Lecithin)	541601	Product Data
Soy Extract (40% Lecithin)	341602	Product Data
Soy PC (95% Lecithin)	441601	Product Data
Polar Extract** (from 20% Lecithin)	541602	Product Data

Samples For Analysis Of Soy Lipid Extracts

Soy Lipid Extract	Total Extract	Polar Extract	95%	40%
Soy Lipid Extract	Cat. No. 541601	Cat. No. 541602	Cat. No. 441601	Cat. No. 341602
Component	Percent Phospholipid	Percent Phospholipid	Percent Phospholipid	Percent Phospholipid
Phosphatidylethanolamine	18.06	22.1	-	16
Phosphatidylinositol	11.5	18.4	-	11
Phosphatidylcholine	24.0	45.7	97.2	40
Phosphatidic Acid	4.3	6.9	-	-
LPC	4.6	-	2.8	-
Other	37.0	6.9	-	33

Note
*Total Lipid Extract is a chloroform:methanol extract of the respective tissue (Brain, Soybean, Heart, etc.). This extract is partitioned against deionized water, and the chloroform phase is concentrated.
** Polar Lipid Extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.