LIPOSOMES

Drawing of vesicle kindly provided by
Dr. André Pampel
Universität Leipzig
Fakultät für Physik und Geowissenschaften

• Formulation and preparation
  of lipid supensions
  
  
• Making preformed vesicles
  
  
• Liposome characterization techniques:
  • Light scattering
  • Fluorescent spectroscopy
  • Phosphorus/Nitrogen content
  • HPLC
    
    
• Lyophilized lipid mixtures
  
  
• Formulations to encapsulate
  hydrophilic drugs
  
  
• Formulations to suspend
  hydrophobic drugs
  
  
• Lipids for Liposome Formation
  

FAQ - Liposomes

• How should I store my liposomes?
• Can I freeze my liposomes?
• How long can I store liposomes?
• What are the differences between liposomes and micelles?
• How do I determine the size of my liposomes?
• What issues do I need to consider when selecting lipids?
• What is an SUV and LUV and how do they differ?
• Why doesn't my SUV prep clear when I sonicate?
• How do I concentrate a liposome?

• Preparation of Liposomes
• Lipids for Liposome Formation
• Preparation of Multilamellar Liposomes (LMV)
• Preparing Large, Unilamellar Vesicles by Extrusion (LUVET)

• Useful Reference Texts