Avanti Polar Lipids

Cationic Transfection Reagents

Lipotransfer

An Alternative to Microinjection/Electroporation Rapid and Non-
Disruptive Delivery of Cellular Probes to Large Populations

An f-actin probe, fluorescent phallacidin, was loaded into 40-50nm cationic liposomes and delivered into living nerve cells. All predicted f-actin structures were flourescent, indicating a high efficacy of delivery. Phallacidin alone did not enter living cells, nor was its uptake stimulated by the presence of empty liposomes. Prominent labeling of living nerve cells occurs along plasma membrane and in cytoplasmic fibers within cell body and processes. Intense labeling of actin bundles in the growth cones, as well as stress and cytoplasmic fibers were observed. Cationic lipotransfer of fluorescent probes was rapid, not disruptive to cells, and delivered a probe en masse to a large sample population. Lipotransfer did not effect the cell morphology.

Photo courtesy of Professor William L. Klein, Ph.D., Northwestern University, Evanston, IL

Lipotransfer Lipids

Ethyl PC

Purity >99%

1,2-Diacyl-sn-Glycero-3-Ethylphosphocholine, Chloride Salt

Chain	Acyl Group	M.W.	Catalog Number
12:0	Dilauroyl	686.35	890700	Product Data
14:0	Dimyristoyl	742.46	890701	Product Data
16:0	Dipalmitoyl	798.57	890702	Product Data
18:0	Distearoyl	854.67	890703	Product Data
18:1	Dioleoyl (DOPC+)	850.64	890704	Product Data
16:0-18:1	Palmitoyl-Oleoyl	824.60	890705	Product Data

Specify Chloroform or Powder for these products

Ethyl DPPC as a Transfection Agent: Effect of SonoPoration^TM on Transfection Rates
FluoroGene is a new class of synthetic delivery vehicles with a fluorinated core that efficiently complexes genes into stable submicron-sized particles. SonoPoration enhances gene expression and localization with ultrasound. In these in vivo studies FluoroGene (made with Avanti's Ethyl DPPC) and SonoPoration were used to deliver the CAT gene via IM and IV injections in mice. SonoPoration preferentially increased gene expression in tissues that were exposed to ultrasound, and IV injection of FluoroGene caused widespread CAT gene expression with high levels of expression in kidney, in spleen, and in muscle. FluoroGene works synergistically with SonoPoration, and levels of gene expression appear to be higher than with other synthetic delivery vehicles.*
*Unger, E.C., "FluoroGene^TM and SonoPoration^TM Gene Delivery" (1997) Presented at the Cambridge Healthtech Institute's 4th Annual Meeting, Oct 12-14, 1997, by ImaRx Pharmaceutical Corporation.

References:

Barber, K., Mala, R.R., Lambert, M.P., Qiu, R., MacDonald, R.C., Klein, W.L.; (1996), "Delivery of membrane-impermeant fluorescent probes into living neural cell populations by lipotransfer," Neuroscience Letters 207,1720.
MacDonald, R.C., G.W. Ashley, M.M. Shida, V.A. Rakhmanova, Y.S. Tarahovsky, D.P. Pantazatos, M.T. Kennedy, E.V. Pozharski, K.A. Baker, R.D. Jones, H.S. Rosenzweig, K.L. Choi, R. Qiu, and T.J. McIntosh. (1999). Physical and biological properties of cationic triesters of phosphatidylcholine. Biophys J 77:2612-29.
MacDonald, R.C., V.A. Rakhmanova, K.L. Choi, H.S. Rosenzweig, and M.K. Lahiri. (1999). O-ethylphosphatidylcholine: A metabolizable cationic phospholipid which is a serum-compatible DNA transfection agent. J Pharm Sci 88:896-904.
Matsumura, J.S., R. Kim, V.P. Shively, R.C. MacDonald, and W.H. Pearce. (1999). Characterization of vascular gene transfer using a novel cationic lipid. J Surg Res 85:339-45.