Avanti Polar Lipids

700 Industrial Park Drive
Alabaster, AL 35007
1-800-227-0651 or 205-663-2494
Fax 1-800-229-1004 or 205-663-0756
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Natural Products

Cholesterol

Purity >98%

Source	M.W.	Physical State	Catalog Number
Cholesterol	386.654	Powder	700000	Product Data

Specifications
	HPLC:	>98% Cholesterol
		<0.2% 25-hydroxycholesterol
Solubility
	Soluble:	Chloroform, Toluene
	Insoluble:	Water, Methanol, Ethanol
Stability
	Storage: -20^°C
	Shelf Life: 12 Months
Preparation
	Cholesterol is prepared from wool grease. The purification of the product includes crystallization, filtration, and drying procedures. The final product is dried in a vacuum oven. Cholesterol is rapidly oxidized in air.
TLC Analysis Procedure
	TLC Plate: Whatman K6 (normal phase silica) Mobile Phase: Hexane:Ethyl Acetate (7:3, v/v) Detection Method: Iodine vapors Rf: ~0.35
Notes
	25-hydroxycholesterol is determined to be less than 0.2% by reverse phase chromatography, and all other oxidation products are less than 2.0%. The purity, as determined by reverse phase chromatography, is >98%.

Reference

Lang, J., (1990), "Quantitative Determination of Cholesterol in Liposome Drug Products and Raw Materials by High Performance Liquid Chromatography," Journal of Chromatography, 507, 157-163. Smith, L., (1981), Cholesterol Autoxidation, Plenum Press, New York.