Natural Products

L-a-Phosphatidylcholine (Tissue-Derived)

Purity >99%

Typical Phosphatidylcholine Structure

L-a-Phosphatidylcholine (Egg, Chicken)

Source M.W. Catalog Number Egg Yolk 760.08* 840051 Product Data Heart (Bovine -Approx. 33% plasmalogen) 758.07‡ 840052 Product Data Brain 760.08* 840053 Product Data Liver (Bovine) 786.12** 840055 Product Data Plant (Soybean) 758.07‡ 840054 Product Data

* Based on Palmitoyl-Oleoyl Phosphatidylcholine. ‡ Based on Palmitoyl-Linoleoyl Phosphatidylcholine. ** Based on Stearoyl-Linoleoyl Phosphatidylcholine. Specifications TLC: One phosphorus and iodine positive spot. Ninhydrin negative. GC: Conforms to appropriate standard HPLC: >99%AUC. Solubility Soluble: Chloroform, Hexane-ethanol, Methanol, Ethanol, Toluene, Ether. Insoluble: Water (CMC < 0.001nM), Cold Acetone. Stability Storage: -20°C Shelf Life: 6 Months Preparation Fresh tissue is extracted with organic solvents; non-lipids are then removed; and finally, lipid classes are fractionated. Pure (>99%) phospha-tidylcholine is isolated using a series of chromatography columns. Oxidation is minimized by removing solvent at low temperatures and back-filling evacuated chambers containing dry lipid with nitrogen gas. The finished product is stored as a chloroform solution in a sealed amber bottle at -20°C.

Notes Natural phospholipids are readily oxidized because, in addition to saturated hydrocarbons, they contain multiple unsaturated hydrocarbons. The linkage between the glycerol and the long-chain hydrocarbon is predominantly the ester bond. In the sn-2 position, the ester bond is the only type of linkage. However, in the sn-1 position of phosphatidylcholine from Liver, Heart, and Brain, several linkages exist: ester-linked hydrocarbons and vinyl ether-linked hydrocarbons (plasmalogen). Reference Yesair, David W., (1990), "Phosphatidyl Choline and Lysophosphatidyl Choline in Mixed Lipid Micelles as Novel Drug Delivery Systems", Phospholipids: Biochemical, pharmaceutical, and Analytical Considerations, (Hanin, Israel and Pepeu, Giancarlo, Ed.) p83, Plenum Press, NY.