| Specifications |
| TLC: |
One phosphorus positive spot; one ninhydrin positive
spot |
| GC: |
Conforms to appropriate standard. |
| HPLC: |
>99% diacyl compounds |
| Solubility |
| Soluble: |
Chloroform, Toluene |
| Insoluble: |
Ethanol, Methanol, Water |
| Stability |
| Storage: |
-20oC |
| Shelf Life: |
3 Months |
Preparation
Phosphatidylserine is prepared from brain
extracts and transphosphatidylation of soybean lecithin.
Product purification includes column chromatography on a
variety of solid supports. The final product is washed with
EDTA and sodium chloride to remove excess calcium and produce
the sodium salt of the compound.
Notes
Phosphatidylserine is rapidly oxidized in
air. The amount of oxidation can be evaluated from the ratio
of the absorption bands at 234 and 215 nm in the UV-spectrum
(Klein, 1970). A ratio of 0.02 corresponds to 0.1% oxidation
products.
Reference
- van den Besselaar, A.M.H.P., Neutenboom, J. & Bertina,
R.M., (1993), "Effect of Synthetic Phospholipids
on the Response of the Activated Partial Thrombo-plastin
Time to Heparin", Blood Coagulation and Fibrinolysis,
4, 895-903
- Klein, R.A., (1970), The detection of oxidation in liposome
preparations, Biochim Biophys Acta, 210, 486
- Mosioer, M. & Epand, R., (1993), "Mechanism
of Activation of Protein Kinase C: Roles of Diolein and
Phosphatidylserine", Biochemistry 31, 66-75.
|
