Avanti Polar Lipids

Natural Products

Sphingomyelin

Purity >99%

Typical Sphingomyelin Structure

(2S,3R,4E)-2-acylaminooctadec-4-ene-3-hydroxy-1-Phosphocholine (Brain, Porcine)

Source	M.W.	Physical State	Catalog Number
Brain	731.09	Powder	860062	Product Data
Egg	703.03	Powder	860061	Product Data
Milk	801.22	Powder	860063	Product Data

Specifications
TLC: One or two phosphorus positive spots

Solubility
Soluble: Chloroform, Methanol, Hot Ethanol, Hot Ethyl Acetate
Insoluble: Ether, Acetone, Water

Stability
Storage: -20^°C
Shelf Life: 2 Years

Preparation
Prepared from the mild alkaline hydrolysis of the appropriate tissue extract. The product is purified by high-pressure liquid chromatography. Sphingomyelin is a white crystalline non-hygroscopic substance, resistant to degradation by air and light.

Note
Avanti offers sphingomyelin from three sources: egg yolk, milk and brain tissue. Sphingomyelin often shows two spots on TLC plates run with chloroform-methanol-water mixtures. The more rapidly moving component contains principally the longer fatty acids. Whereas the slower component contains mainly the shorter fatty acids.

Reference
Wood, P.D.S. and Holton, S., (1964) Proc. Soc. Exptl. Biol. Med.115, 990.

Fatty Acid Content of
Tissue Derived Sphingomyelin

*Note: Results expressed as area percentage by FAME - GC/FID