Comparison of
Quality and Binding Analysis
of S1P and DH-S1P
Reprinted from
“Pharmacological characterization of human S1P4 using a novel radioligand, [4,5-3H]-dihydrosphingosine-1-phosphate”
By James Fossetta, Gregory Deno, Waldemar Gonsiorek, Xuedong Fan, Brian Lavey,
Pradip Das, Charles Lunn, Paul J Zavodny, Daniel Lundell, and R William Hipkin.
The British Journal of Pharmacology (2004); 142: 851-860

[Abstract] [PubMed]

Competition-binding analysis using different commercially available sphingophospholipids.
Membranes (2 µg per well) from Ba/F3-S1P₄-42 cells were incubated in binding buffer at room
temperature (as described in Methods) with 4 nM [³H]DH-S1P and the indicated concentrations
of unlabeled DH-S1P (left) or S1P (right). Radioligand binding to the membranes was measured
by WGA-SPA scintillation. Data represent the mean±s.e.m. of triplicate determinations from a
representative experiment (n=2).