|
Synthetic
Products
|
| Amphipathic
Antigens |
Antigenic
Phosphatidylethanolamines |
|
Purity
>99%
|
|
|
|
Product
|
M.W.
|
Catalog
Number
|
|
|
N-Dinitrophenyl
Phosphatidylethanolamine (DNP-PE) 16:0
|
880.04
|
810508
|
|
Specify Chloroform or Powder
for this product
|
|
|
|
Product
|
M.W.
|
Catalog
Number
|
|
|
N-Dinitrophenylaminocaproyl
Phosphatidylethanolamine (DNP-Cap PE) 16:0
|
993.20
|
810528
|
|
Specify Chloroform or Powder for this
product
|
Specifications
of Antigenic Phosphatidylethanolamines
|
| Physical Examination |
|
TLC: |
One phosphorus positive spot |
|
GC: |
>99% (AUC) of methyl palmitate. |
|
HPLC: |
>99% (AUC) of diacyl lipid |
| Solubility |
|
Soluble: |
chloroform, methanol, toluene |
|
Insoluble: |
acetone, water |
| Stability |
|
Storage: |
-20°C |
|
Shelf Life: |
6 Months |
Note:
Antigenic compounds that can be characterized as "hydrophobic"
are able to sensitize liposomes to the action of antibody
complement. Dinitrophenyl derivatives of phosphatidylethanolamine
and aminocaproyl phosphatidylethanolamine are offered as antigens
for the preparation of immunologically responsive liposomal
model membranes.
References
- Balakrishnan, K., Mehdi, S.Q. & McConnell, H.M., (1982),
"Availability of Dinitrophenylated Lipid Haptens for
Specific Antibody Binding Depends on the Physical Properties
of Host Bilayer Membranes", J. Biol. Chem. 257,
6434-6439.
- Timbs, M.M., Poglitsch, C.L., Pisarchick, M.L., Sumner,
M.T. & Thompson, N.L., (1991), "Binding and Mobility
of Anti-Dinitrophenyl Monoclonal Antibodies on Fluid-like
Langmuir-Blodgett Phospholipid Monolayers Containing Dinitrophenylated
Phospholipids", Biochim. Biophys. Acta 1064,
219-228.
Pisarchick, M.L. & Thompson, N.L., (1990), "Equilibrium
Binding of Monoclonal Antibody to Supported Phospholipid
Monolayers Measured by Total Internal Reflection Fluorescence
Microscopy", Biophys. J. 58, 1235-1249.
- Aragnol D. & Leserman, L.D., (1986), "Immune
Clearance of Liposomes Inhibited by an Anti-FC Receptor
Antibody in vivo", Proc. Nat. Acad. Sci. USA 83,
2699-2703
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