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Synthetic Lipid Mixtures
Coagulation Reagents
Synthetic Phospholipid Blend (Dioleoyl)
Product No 790304 - Product Details
PE:PS:PC (5:3:2, wt%)

Synthetic Phospholipid Blend (Dioleoyl)
Product No 790413 - Product Details
PC:PS (7:3, wt%)

Reference List:

Transfection Mixtures
Cationic Lipid DOTAP

Synthetic Transfection Blend (Dioleoyl)
Product No 790310 - Product Details
DOTAP:DOPE (1:1 wt%)

Synthetic Transfection Blend (Dioleoyl)
Product No 790326 - Product Details
Fluorescent Reagent
DOTAP:DOPE (1:1 wt%)
with 0.1% wt% NBD-DOPE

Reference List:

  1. Lewis, R.N. and R.N. McElhaney. (2000). Surface charge markedly attenuates the nonlamellar phase-forming propensities of lipid bilayer membranes: calorimetric and (31)P-nuclear magnetic resonance studies of mixtures of cationic, anionic, and zwitterionic lipids. Biophys J 79:1455-64.
  2. Wong, G.C., J.X. Tang, A. Lin, Y. Li, P.A. Janmey, and C.R. Safinya. (2000). Hierarchical self-assembly of F-actin and cationic lipid complexes: stacked three-layer tubule networks. Science 288:2035-9.
  3. Macdonald, P.M., K.J. Crowell, C.M. Franzin, P. Mitrakos, and D. Semchyschyn. (2000). 2H NMR and polyelectrolyte-induced domains in lipid bilayers. Solid State Nucl Magn Reson 16:21-36.
  4. Kim, T.W., H. Chung, I.C. Kwon, H.C. Sung, and S.Y. Jeong. (2000). In vivo gene transfer to the mouse nasal cavity mucosa using a stable cationic lipid emulsion. Mol Cells 10:142-7.
  5. Meidan, V.M., J.S. Cohen, N. Amariglio, D. Hirsch-Lerner, and Y. Barenholz. (2000). Interaction of oligonucleotides with cationic lipids: the relationship between electrostatics, hydration and state of aggregation. Biochim Biophys Acta 1464:251-61.
  6. Simoes, S., V. Slepushkin, P. Pires, R. Gaspar, M.C. Pedroso de Lima, and N. Duzgunes. (2000). Human serum albumin enhances DNA transfection by lipoplexes and confers resistance to inhibition by serum. Biochim Biophys Acta 1463:459-69.
  7. Mitrakos, P. and P.M. Macdonald. (2000). Nucleotide chain length and the morphology of complexes with cationic amphiphiles: (31)P-NMR observations. Biochim Biophys Acta 1463:355-73.
  8. Urtti, A., J. Polansky, G.M. Lui, and F.C. Szoka. (2000). Gene delivery and expression in human retinal pigment epithelial cells: effects of synthetic carriers, serum, extracellular matrix and viral promoters. J Drug Target 7:413-21.
  9. Lin, A.J., N.L. Slack, A. Ahmad, I. Koltover, C.X. George, C.E. Samuel, and C.R. Safinya. (2000). Structure and structure-function studies of lipid/plasmid DNA complexes. J Drug Target 8:13-27.
  10. Hirsch-Lerner, D. and Y. Barenholz. (1999). Hydration of lipoplexes commonly used in gene delivery: follow-up by laurdan fluorescence changes and quantification by differential scanning calorimetry. Biochim Biophys Acta 1461:47-57.
  11. Zuidam, N.J., Y. Barenholz, and A. Minsky. (1999). Chiral DNA packaging in DNA-cationic liposome assemblies. FEBS Lett 457:419-22.
  12. Zuidam, N.J., D. Hirsch-Lerner, S. Margulies, and Y. Barenholz. (1999). Lamellarity of cationic liposomes and mode of preparation of lipoplexes affect transfection efficiency. Biochim Biophys Acta 1419:207-20.
  13. Xu, Y., S.W. Hui, P. Frederik, and F.C. Szoka Jr. (1999). Physicochemical characterization and purification of cationic lipoplexes. Biophys J 77:341-53.
  14. Zuidam, N.J. and Y. Barenholz. (1999). Characterization of DNA-lipid complexes commonly used for gene delivery. Int J Pharm 183:43-6.
  15. Pires, P., S. Simoes, S. Nir, R. Gaspar, N. Duzgunes, and M.C. Pedroso de Lima. (1999). Interaction of cationic liposomes and their DNA complexes with monocytic leukemia cells. Biochim Biophys Acta 1418:71-84.
  16. Ross, P.C. and S.W. Hui. (1999). Lipoplex size is a major determinant of in vitro lipofection efficiency. Gene Ther 6:651-9.
  17. Koltover, I., T. Salditt, J.O. Radler, and C.R. Safinya. (1998). An inverted hexagonal phase of cationic liposome-DNA complexes related to DNA release and delivery. Science 281:78-81.
  18. Hirsch-Lerner, D. and Y. Barenholz. (1998). Probing DNA-cationic lipid interactions with the fluorophore trimethylammonium diphenyl-hexatriene (TMADPH). Biochim Biophys Acta 1370:17-30.
  19. Zelphati, O., L.S. Uyechi, L.G. Barron, and F.C. Szoka Jr. (1998). Effect of serum components on the physico-chemical properties of cationic lipid/oligonucleotide complexes and on their interactions with cells. Biochim Biophys Acta 1390:119-33.
  20. Barron, L.G., K.B. Meyer, and F.C. Szoka Jr. (1998). Effects of complement depletion on the pharmacokinetics and gene delivery mediated by cationic lipid-DNA complexes. Hum Gene Ther 9:315-23.
  21. Zuidam, N.J. and Y. Barenholz. (1998). Electrostatic and structural properties of complexes involving plasmid DNA and cationic lipids commonly used for gene delivery. Biochim Biophys Acta 1368:115-28.
  22. Bauchwitz, R. and F. Costantini. (1998). YAC transgenesis: a study of conditions to protect YAC DNA from breakage and a protocol for transfection. Biochim Biophys Acta 1401:21-37.
  23. Zuidam, N.J. and Y. Barenholz. (1997). Electrostatic parameters of cationic liposomes commonly used for gene delivery as determined by 4-heptadecyl-7-hydroxycoumarin. Biochim Biophys Acta 1329:211-22.
  24. Templeton, N.S., D.D. Lasic, P.M. Frederik, H.H. Strey, D.D. Roberts, and G.N. Pavlakis. (1997). Improved DNA: liposome complexes for increased systemic delivery and gene expression. Nat Biotechnol 15:647-52.
  25. Radler, J.O., I. Koltover, T. Salditt, and C.R. Safinya. (1997). Structure of DNA-cationic liposome complexes: DNA intercalation in multilamellar membranes in distinct interhelical packing regimes. Science 275:810-4.
    Notes: COMMENTS: Comment in: Science 1997 Feb 7;275(5301):791-2
  26. Hui, S.W., M. Langner, Y.L. Zhao, P. Ross, E. Hurley, and K. Chan. (1996). The role of helper lipids in cationic liposome-mediated gene transfer. Biophys J 71:590-9.
Cationic Lipid DC CHOL

Synthetic Transfection Blend
(DC-CHOL)

Product No 790625 - Product Details
DC-CHOL:DOPE (3:7 wt%)

Reference List:
  1. Son, K.K., D. Tkach, and D.H. Patel. (2000). Zeta potential of transfection complexes formed in serum-free medium can predict in vitro gene transfer efficiency of transfection reagent. Biochim Biophys Acta 1468:11-4.
  2. Kawakami, S., A. Sato, M. Nishikawa, F. Yamashita, and M. Hashida. (2000). Mannose receptor-mediated gene transfer into macrophages using novel mannosylated cationic liposomes. Gene Ther 7:292-9.
  3. Hirsch-Lerner, D. and Y. Barenholz. (1999). Hydration of lipoplexes commonly used in gene delivery: follow-up by laurdan fluorescence changes and quantification by differential scanning calorimetry. Biochim Biophys Acta 1461:47-57.
  4. Yang, K., X.S. Mu, R.L. Hayes, Y.H. Qiu, F.L. Sorgi, L. Huang, and G.L. Clifton. (1997). DC-Chol liposome-mediated gene transfer in rat spinal cord. Neuroreport 8:2355-8.
  5. McQuillin, A., K.D. Murray, C.J. Etheridge, L. Stewart, R.G. Cooper, P.M. Brett, A.D. Miller, and H.M. Gurling. (1997). Optimization of liposome mediated transfection of a neuronal cell line. Neuroreport 8:1481-4.


Synthetic Phospholipid Blend (Dioleoyl)
Product No 790595 - Product Details
DOPC:DOPS (1:1 wt%)
To Support The Following Assay Kits by Upstate Biotechnology
Follow the Links Below and See
The Latest (largest number)Certificate Of Analysis

Catalog No. 14-279 - Akt1/PKB alpha, unactive Serine/Threonine Phosphorylation
Catalog No. 14-280 - PDK1, active Serine/Threonine Phosphorylation
Catalog No. 14-300 - PTEN Regulators of Lipid Signaling
Catalog No. 17-262
- PTEN Malachite Green Assay Kit Regulators of Lipid Signaling
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