Alabaster, AL 35007
1-800-227-0651 or 205-663-2494
Fax 1-800-229-1004 or 205-663-0756
Orders:
Technical Questions:
General Information:
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Synthetic Products
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| Nickel-Chelating Lipids |
| Two-Dimensional
Crystallization of Histidine-Tagged Peptides and Proteins Using Nickel-Chelating Lipids |
| Proteins containing a short sequence of adjacent histidine residues (~6; "His-tag") can be immobilized on a two dimensional lipid film containing a nickel-chelating group, N",N"-bis[carboxymethyl]-L-lysine (nitriloacetic acid) (NTA), covalently attached to the lipid molecules. NTA-lipids provide a general approach to two-dimensional crystallization of His-tagged proteins for structure determination using electron microscopy and image processing. The technique is rapid, with crystals forming within minutes to hours from dilute protein solutions, and requires only small quantities of material. The use of NTA-lipids has the potential advantage of binding proteins in a unique orientation and allowing crystal formation under a wide range of solution conditions, including physiological conditions. |
| DOGS-NTA-Ni |
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1,2-Dioleoyl-sn-Glycero-3-{[N(5-Amino-1-Carboxypentyl)iminodiAcetic
Acid]Succinyl}
(Nickel Salt) Product Number - 790404 |
| DOGS-NTA |
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1,2-Dioleoyl-sn-Glycero-3-{[N(5-Amino-1-Carboxypentyl)iminodiAcetic
Acid]succinyl}
(Ammonium Salt) Product Number - 790528 |
| 2D Protein Crystallization
His6-Protein solution is placed in a Teflon well. The
surface of the solution is coated with NTA-lipids and various "helper"
lipids. The device is incubated for 3-48 hours at room temperature
in a closed humid chamber. After negative staining the crystals
are examined in an electron microscope. |
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References
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Nickel-Chelating
& "Helper" Lipids
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